LIST OF EQUIPMENTS OF GMO DEPARTMENT

Main Equipments

Real-time PCR machines CFX96- Biorad

Used to detect or quantify the sample by amplified gene sequence

Supported Equipments

Scientific NanoDrop 1000 -Thermo machine

Perform the analysis auxiliary for Western blot, Quantitative and quantitative PCR.

Measuring the concentration of DNA, RNA, Protein.

Syngene gel cameras

Agarose gel DNA capture

Coomassie staining protein shooting

 

Electrophoresis and transfer system proteins - Biorad

Perform technical analysis for Western blot:

Protein electrophoresis, transfer proteins from gel to membrane

Nitrocellulose or PVDF in protein expression analysis

 

Centrifuge Mikro 200R-Hettich

Perform the analysis auxiliary for Western blot,

in the analysis of gene expression and protein.

Auxiliary for quantitative and quantitative PCR.

Centrifuge samples for protein extraction, extraction of DNA, RNA.

Sanyo oven

Use to dry and sterilize laboratory instruments

SANYO refrigerators

Containers and stored at 4 ° C the chemical requirements


Biological Safety Cabinets 2 Esco level

For extracting and preparing the tube PCR reactions for and RT-PCR

 

Hirayama autoclaving HVE-50

Use to sterilize instruments, environmental, accessories and equipment

 

Loopster Digital Camcorder - IKA

Perform analysis to support Western blot

Protein extraction, the first antibody permeability, 2nd antibody in protein expression analysis

 

Scotsman Flake Ice Machine

Providing flake ice for protein extraction process, RNA and several steps of separation, extraction of DNA

 

LabNe tBlock Heat

Heat Shock Protein before electrophoresis and a number of steps to extract the DNA, RNA, protein.